非水反相高效液相色谱法分离测定枸杞子中的类胡萝卜素

Separation and Determination of Carotenoids in Fructus Lycii by Isocratic Non Aqueous Reversed Phase Liquid Chromatography

采用非水反相高效液相色谱法在ＯＤＳ柱上比较了不同流动相系统对枸杞子中类胡萝卜素的分离效果，探索了枸杞子中类胡萝卜素的最佳分离条件。

High performance liquid chromatography using a non aqueous reversed phase with isocratic elution of C 18 columns is a powerful tool for investigating the carotenoid composition of Fructus Lycii . This paper compared the effect of different eluents on the separation of carotenoids. An optimum solvent system consisting of acetonitrile and methylene chloride is suitable for simultaneous separation of compounds from polar xanthophylls to nonpolar carotenoid esters. Ten carotenoids were separated from Fructus Lycii by using Shim pack CLC ODS column(150mm×6mm i.d.) and mobile phase of acetonitrile/methylene chloride(60∶42, V/V ) with a flow rate of 1mL/min. The eluates were monitored with UV/VIS detector at 456nm. The method which permit direct analysis of carotenoid extracts without saponification in 30min provides a true information of carotenoids composition in Fructus Lycii as they exist. Fructus Lycii contains 2952μg/g of total carotenoids, but 98 6% of carotenoids are existed as esterified forms, zeaxanthin dipalmitate are accounted for 77 5% of the total carotenoide.