茜素红S与蛋白质的反应机理研究

Study on the Reaction between Alizarin Red S and Protein

应用ＵＶ光谱法研究在ＰＨ４．３５的缓冲溶液中，茜素红Ｓ（ＡＲＳ）与牛血清蛋白（ＢＳＡ）间的相互结合反应，测定了平均结合数ｎ＝８，分配结合常数ＫＣ＝１．５×１０＾５，研究了小分子探针与蛋白质的反应机理，相互间的结合部位及结合力，该复合物结合前后手等吸收波长红移２８ｎｍ这是目前此类结合反应研究中，其分子间结合力较大的一例，进一步研究了ＡＲＳ与多种蛋白质的结合反应，以及离子强度对该体系的影响。

The interaction of alizarin red S and bovine serum albumin was studied by UV spectra in pH4.35 buffer solution.The binding number(n =8) and binding constant (Kc= 1.5×105) o the probe and protein were obtained. The main sort of binding force was investigated. It was founc that the red shift of isosbestic point of the complex was 28nm and the sodium chloride concentration had significant effect on the binding reaction. The 'Phase Distribution Model' is the proper description of the interaction between alizarin S and the protein.