补肾益精口服液对大鼠生精细胞损伤的保护

Effect of Bushen Yijing(补肾益精) Oral Fluid on Nitric Oxide Synthetase and Antioxidation System of Testis Tissue in Rat with Impaired Spermatogenic Cells

目的:观察补肾益精口服液对生精细胞损伤模型大鼠睾丸组织一氧化氮合酶和抗氧化系统的影响。方法:以腺嘌呤灌胃SD大鼠,诱发生精细胞损伤大鼠模型。测定服用补肾益精口服液前后大鼠睾丸组织超氧化物歧化酶、一氧化氮合酶活性及一氧化氮、丙二醛含量。结果:补肾益精口服液可提高生精细胞损伤大鼠睾丸组织超氧化物歧化酶活性,降低一氧化氮合酶活性及一氧化氮、丙二醛含量。结论:补肾益精口服液对腺嘌呤诱发的大鼠生精细胞损伤具有保护作用。

Ding Caifei, Li Haisong, Wan Lingqi, et al（Dept. of Reproductive Medicine, Zhejiang Provincial Hospital of Integrated Traditional Chinese and Western Medicine, Hangzhou （310003）, China Abstract：Objective To explore the mechanism of Bushen Yijing oral fluid in treating male infertility and its effecton the nitricoxide synthase and antioxidation system of testis tissue. Method The rat model with impaired spermatogenie cell was established by lavaging SD rat with adenine. The levels of superoxide dismutase, mitricoxide synthase, nitrogen monoxidum and malonaldehyde were measured in rat testis tissue before and after administrating Bushen Yijing oral fluid. Results The superoxide dismutase level in testis tissue of model rat was increased while the nitricoxide synthase, nitrogen monoxidum and malonaldehyde levels were decreased after administration with Bushen yijing oral fluid. Conclusion Bushen Yijing oral fluid has a protective effect on rat with impaired spermatogenic cell induced by adenine.