核因子κB在HGF介导的人胎儿肝干细胞增殖分化作用中的变化及意义

The significance on NF-κB changes for HGF-mediated proliferation and differentiation in human fetal hepatic stem cells

目的探讨在由HGF介导的人胎儿肝干细胞增殖分化过程中核因子κB(NF-κB)的变化情况及其意义。方法通过ELASA、免疫细胞化学染色及RT-PCR方法,检测在不同浓度HGF作用条件下,人胎儿肝干细胞中NF-κB活性改变情况。结果HGF可促进人胎儿肝干细胞的增殖与分化,在此过程中,HGF浓度与NF-κB活性呈现正相关;免疫细胞化学染色显示,随着HGF剂量的增加,hFHSCs细胞胞核染色程度及出现核染色的细胞的数量亦逐渐增加。RT-PCR结果显示,HGF可促进人胎儿肝干细胞P65及P50mRNA表达,下调IκBmRNA表达,从而使IκB生成减少,促进NF-κB核移位。结论NF-κB信号转导途径可能在肝再生过程中发挥重要作用。

ObjectiveTo explore the significance on NF - κB changes for HGF - mediated proliferation and differentiation in human fetal hepatic stem cells （hFHSCs）. Methods By ELASA, immunocytochemistry staining and RT - PCR methods used, the NF - κB activity changes in hFHSCs were detected for the role of HGF concentrations in different conditions. Resuits HGF can promote the proliferation and differentiation of hFHSCs, in the process, and NF - κB activity showed a positive correlation with HGF concentrations; immunocytochemical show that with the increase in HGF dose, the staining degree of cells nucleus and the number of the ceils whose nucleus were stained were both gradually increase. RT - PCR showed that HGF can promote the expression of hFHSCs P65 and PSOmRNA, I K BmRNA expression was reduced, so that the production of I K B was deseased, which promote nuclear translocation of NF - KB. Conclusion NF - κB signal transduction pathways in the process of liver regeneration may play an important role.