不同类型慢性乙肝病毒感染者外周血树突状前体细胞亚群的变化特点

Characteristics of circulating precursor dendritic cell subsets in patients with different types of chronic Hepatitis B virus infection

目的观察不同类型慢性乙肝病毒感染者外周血树突状前体细胞(pDC)亚群的变化,探讨pDC亚群在慢性乙肝病毒感染自然史中的意义。方法采集健康对照组15例,免疫耐受组19例,慢性乙型肝炎组26例,非活动性组33例的外周血,用流式细胞技术检测髓样树突状前体细胞(pDC1)和浆细胞样树突状前体细胞(pDC2)。结果与健康对照组比较,慢性乙型肝炎组pDC2显著下降(P<0.05),慢性乙型肝炎组pDC1显著高于免疫耐受组(P<0.05),非活动性组pDC2显著高于免疫耐受组和慢性乙型肝炎组(P<0.05)。相关性分析表明,HBVDNA水平与pDC2之间存在正相关(r=0.303,P=0.043)。结论慢性乙肝病毒感染自然史不同阶段的外周血树突状前体细胞(pDC)亚群的频率和数量的变化,可能与HBV持续感染的机制和肝炎的发病机制有关。

Objective To investigate the characteristics of circulating precursor dendritic cell subsets （pDC） in patients with different infection and discuss its clinical significances in the natural history of the chronic HBV infection. Methods Fifteen healthy individuals were enrolled as controls, 19 patients with immune torlerence and 26 patients with chronic hepatitis B and 33 patients with inactive hepatitis B were included in our study. The frequencies of circulating myloid precursor dendritic cells （pDC1） and lymphoid precursor dendritic ceils （pDC2） in peripheral blood mononuclear cells （PBMC） were detected by flow cytometry. Results Compared with healthy controls, the percentage of pDC2 significantly decreased （ P 〈 0. 05 ） in chronic hepatitis B. The percentage of pDC1 in chronic hepatitis B was significantly higher than that in immune tolerence goup （ P 〈 0.05 ）. The percentage of pDC2 in inactive hepatitis B group was significantly higher than that in immune tolerence group and in chronic hepatitis B group （ P 〈 0 . 05 ）. In addition, serum HBV - DNA levels were significantly correlated positively with the pDC2 percentage （ r = 0. 303, P = 0. 043 ）. Conclusion The characteristics of circulating precursor dendritic cell subsets （pDCs） in different phases of natural history of hepatitis B, which may contribute to the persistence of HBV infection and the pathogenesis of hepatitis B.