5-羟色胺2C受体基因启动子区-759C/T多态性与甘肃东乡族2型糖尿病的关系

Association of-759C/T polymorphism of 5-HT2C receptor gene with type 2 diabetes in Gansu Dongxiang nationality

目的:探讨5-羟色胺2C受体(HTR2C)基因启动子区-759C/T多态性与甘肃东乡族2型糖尿病(T2DM)和肥胖的相关性.方法:应用PCR-连接酶检测反应(PCR-LDR)方法检测100名T2DM患者(T2DM组)和126名健康对照者(NC组)HTR2C基因-759C/T多态性,并测量身高、体质量、腰围、臀围、血压、血脂、空腹血糖及空腹胰岛素.结果:①女性NC组和T2DM组CC,CT基因型频率分别为61%,39%,80.6%,19.4%,T2DM组CT基因型频率明显低于NC组(P=0.047);两组C,T等位基因频率分布差异无统计学意义(P=0.073);男性NC组和T2DM组C,T半合子型频率差异无统计学意义(P=0.727);②女性CC,CT基因型及C,T等位基因频率和男性C,T半合子型频率在非肥胖组和肥胖组差异无统计学意义.结论:HTR2C基因启动子区-759C/T多态性与甘肃东乡族女性T2DM相关联,-759C→T单核苷酸置换可能是防止女性发生T2DM的基因保护性因子.

AIM： To study the association of - 759C/T polymorphism of 5-HT2C receptor （HTR2C） gene with type 2 diabetes and obesity in Gansu Dongxiang nationality. METHODS： HTR2C gene -759C/T polymorphism was detected by PCR- ligase detection reaction （PCR-LDR） technique in 100 type 2 diabetics and 126 normal control subjects. The clinical parameters and serum markers were measured. RESULTS： The frequency of CC and CT genotypes in female diabetics and normal control subjects were 80. 6% and 19.4%, and 61% and 39% respectively. The frequency of CT genotype was significantly lower in the female diabetics than that in the normal control subjects （P = 0.047 ） and the allele frequencies showed no statistical difference between the two groups （P = 0.073 ）. The frequency of C and T hemizygote showed no statistical difference between male diabetics and normal control subjects （ P = 0. 727 ）. The genotype and allele frequencies of -759C/T were not significantly different between female obese group and non-obese group （ P 〉 0.05 ）. The frequency of C and T hemizygote showed no significant difference between male obese group and non-obese group. CONCLUSION： The HTR2C gene -759C/T polymorphism is associated with type 2 diabetes in female Dongxiang nationality. -759C→T may be a protective factor for the development of T2DM.