多孔n-HA/PA66支架复合rhBMP-2修复兔桡骨缺损的实验研究

An Experimental Study on Using the Porous Composite of Nano-hydroxyapatite and Polymide as Scaffold Compound with rhBMP-2 to Repairing Bone Defect on Rabbits

目的:研究多孔纳米羟基磷灰石/聚酰胺66(n-HA/PA66)骨修复材料作为骨组织工程支架复合基因重组人骨形态发生蛋白2(rhBMP-2)后的成骨能力的变化,探讨加速n-HA/PA66人工骨与受体骨愈合的方法。方法:选用新西兰大白兔双侧桡骨制作骨缺损模型,将n-HA/PA66/rhBMP-2复合材料植入左侧骨缺损处,右侧骨缺损以n-HA/PA66植入作为实验对照,另做不植入任何材料的骨缺损空白对照。在1、2、4、8、12周各时相点分别进行大体观察、X线照片、组织学切片、免疫组化原位杂交进行检测图象分析。结果:n-HA/PA66/BMP2与n-HA/PA66组骨缺损均完全修复,而空白对照组骨缺损未见修复;2周时n-HA/PA66与n-HA/PA66/rhBMP-2两组间原位杂交阳性细胞表达有统计学意义(P<0.05),4周时n-HA/PA66与n-HA/PA66/rhBMP-2两组间原位杂交阳性细胞表达无统计学意义(P>0.05),2周及4周实验和实验对照两组分别与空白对照组比较均无统计学意义(P>0.05),n-HA/PA66/rhBMP-2组较n-HA/PA66组可加速人工骨/植入体/受体界面骨愈合。结论:多孔n-HA/PA66作为骨组织工程支架复合具有诱导成骨活性的rhBMP-2后,增强了早期成骨能力,加速了其与受体骨的愈合。

Objective：To evaluate the ability of the porous nano-hydroapatite crystals and polyamide composite （n-HA/PA66） as scaffold compound with rhBMP-2 to repairing bone defect, study the methods which fast the artificial bone with host bone healing in vivo. Methods ： The animal models of bilaterial radius bone defect created with surgery in the New Zealand white rabbits, and were implanted with n-HA/PA66/ rhBMP-2 as experimental group, implanted n-HA/PA66 as experimental control group, and no implant as blank control group. The effect were observed by gross X-ray and histopathlogical examination and in situ hybridization atl, 2, 4, 8, 12W after operation, and use the spssl2.0 and One-Way-ANOVA system to analysis. Results： The defects of experimental group and experimental control group were repair perfectly, The blank control group hadn＇t repaired after 12weeks, the speed of bone-healing of n-HA/PA66/rhBMP-2 group were faster than n-HA/PA66 group in the early time. The data of in situ hybridization analysis had statistically significant difference between n -HA/PA66/rhBMP-2 and n-HA/PA66 group in two weeks, but the data had no statistically significant difference between n-HA/PA66/ rhBMP-2 and n-HA/PA66 group in four weeks . Conclusion：The results show porous scaffold of n-HA/PA66 compound with rhBMP-2 can enhance the ability of the artificial bone with host bone healing in the early time in vivo.