黑曲霉生产菊粉酶工艺条件的研究

The Study on Fermentation Conditions of Inulase Produced by Aspergillus niger

从徐州市沛县河口镇秦庄村牛蒡种植基地取得的土壤样本中,筛选出产菊粉酶的菌株,对从土壤中分离到的40株产菊粉酶的各类微生物进行酶活的测定。通过透明圈法初筛及摇瓶复筛,获得产菊粉酶能力较高的霉菌菌株3株,为C122803、D081506和D081513,这3株菌株的酶活分别达到:C122803:1.411U/ml;D081506:1.895U/ml;D081513:1.792U/ml。其中D081506的酶活最高,为1.895U/ml;对D081506号黑曲霉产菊粉酶的发酵条件进行了研究,确定了优化的发酵条件为:牛蒡汁2%,酵母膏1.6%,(NH4)2SO41.6%,NaCl0.5%,K2HPO40.5%,pH5.0,在27℃、140r/min条件下,摇瓶培养24h,D081506酶活力为2.958U/ml,酶活力提高56.09%。

The high production inulase strain was screened from the soil sample where burdock planted in Qin Village, Bayou Town, Pei County, Xuzhou. Inulase activity were determined which produced by 40 strains separated from soil. Three mold stains, C122803,D081506 and D081513, which had higher ability of producing inulase were obtained by using transparent circle method as initial screening and rocker method as re-screening. Enzyme activity of the three strains were 1. 411U/ml, 1. 895U/ml, 1. 792U/ml, separately . Enzyme activity of D081506, 1. 895U/ml, was the highest. The fermentation conditions of D081506 were studied and the optimized conditions were lappa juice 2.0% , yeast extraction 1.6% , （NH4 ）2SO4 0.5% , NaC1 0.5% , KEHPO4 0.5% and pH 5.0. Inulase activity of D081506 was 2. 9578U/ml which increased 56.09% under the condition of 27℃ ,140r/min, 24h.