多肽串联基因构建策略

The Construction Strategy for Tandem Polypeptide Genes

构建串联表达载体可有效提高小分子重组蛋白(肽)表达量和结构稳定性,并能屏蔽毒蛋白对宿主的伤害作用,因而被广泛采用。比较分析和综述了非对称粘性末端互补法、接头连接法、同尾酶法、表达盒串联法等4种多聚体、多拷贝基因串联表达载体的构建方法特点和适用范围。从效率、精度和产物切割等方面阐述了串联表达载体构建策略的选择依据。

The tandem expression vector can effectively increase the expression level and structure stability of the target peptides （protein） with small size of molecular, and avoid toxicity towards host cell from some toxic peptides product, therefore, this approach is widely applied in biotechnology. The four construction methods of tandem expression plasmid including asymmetric and complementary cohesive ends, directional adapter, isocaudarners, and tandem expression cassette were reviewed in terms of protocol, characteristic and applicable field. In addition, selection principles from various construction methods of tandem plasmid were reviewed in terms of efficiency, accuracy and product cleavage.