DNA分子量标准制备技术:方法与进展

Preparation of DNA Molecular Weight Standards:Methods and Advances

DNA分子量标准是一组分子量大小已知的DNA片段混合物,用于指示核酸电泳中未知样品的分子量大小,从而帮助实验人员判断DNA样品的性质。因而DNA分子量标准成为目前分子生物学和基因工程领域不可或缺的一种电泳耗材。综述了目前各种DNA分子量标准产品的制备方法和技术原理及近年来该领域的一些技术进展情况。

DNA molecular weight standards are a mixture of a grou Pof DNA molecules with known sizes to reference the molecular weight （mass） of unknown DNA samples in nucleic acid electrophoresis and hel Pthe experimentalists to judge the property of the DNA samples. At the present time the DNA molecular weight standards are essential for nucleic acid electrophoresis analysis in the molecular biology and genetic engineering fields. The methods and principles for the production of DNA molecular weight standards with the recent advances in this field were summarized.