肺癌与人乳头状瘤病毒感染和胰岛素样生长因子Ⅱ蛋白表达的相关性及其临床意义

The relationship between HPV infection and the expression of insulin-like growth factor Ⅱ in lung cancer and its clinical significance

目的:研究肺癌发生、发展中人乳头状瘤病毒(human papillomavirus,HPV)感染的病因学意义以及胰岛素样生长因子Ⅱ(insulin-like growth factor-Ⅱ,IGF-Ⅱ)蛋白的表达,并探讨肺癌发生与这2者之间的相关性。方法:采用PCR和免疫组织化学SP法分别检测68例肺癌组织和12例肺良性病变组织中HPV16/18型DNA?高危广谱型HPVE6/E7原癌蛋白和IGF-Ⅱ蛋白的表达水平。结果:肺癌组织的HPV阳性检出率为44.1%,高于肺良性病变组织的8.3%,差异有统计学意义(P<0.05)。HPV感染仅与肿瘤分化程度相关。IGF-Ⅱ蛋白在肺癌组织中的表达水平明显高于肺良性病变组织,且与肿瘤分化程度?淋巴结转移和临床分期相关(P<0.05)。肺癌组织HPV阳性组与阴性组IGF-Ⅱ蛋白的表达水平差异有统计学意义(P<0.05),HPV阳性组中IGF-Ⅱ蛋白的表达水平较高。结论:高危HPV16/18型感染在肺癌的发生中具有重要的病因学意义,并可能通过整合的HPVE6/E7原癌蛋白促使有丝分裂原IGF-Ⅱ高表达,从而在肺癌的发生、发展中发挥重要作用。

Objective：To investigate the etiological role of human papillomavirus（HPV） infection and the expression of insulin-like growth factor Ⅱ（IGF-Ⅱ）in the carcinogenesis of lung cancer and to elucidate the possible correlation of the high expression of IGF-Ⅱ and HPV infection with lung cancer.Methods： Polymerase chain reaction（PCR） was used to detect the HPV 16/18 type DNA and SP immunohistochemical（IHC） method was used to determine the expression of high-risk spectrum HPV E6/E7 oncoproteins and IGF-Ⅱ protein in 68 cases of lung cancer and 12 cases of benign lung diseases.Results： The positive rate of HPV infection was 44.1%（30/68） in the lung cancer tissues,which was higher than that in the tissues of lung benign disease（8.3%）.There was a statistical difference between two groups（P〈0.05）.The infection of HPV was only related to the differentiation grade of lung cancer.The expression degree of IGF-Ⅱ was statistically higher in lung cancer tissues than that in the tissues of lung benign diseases,and it was rela-ted with the differentiation grade,the lymph node metastasis,and the clinical stage（P〈0.05）.In addition,the expression degree of IGF-Ⅱ was statistically higher in the HPV-positive group than that in the HPV-negative group.Conclusion： High-risk HPV 16/18 infection has an important etiological significance in the tumorigenesis of lung cancer.It may stimulate the overexpression of mitogen IGF-Ⅱ through secretion of integrated HPV E6/E7 oncoprotein,thereby play an important role in the initiation and progression of lung cancer.