老年人牙周膜细胞附着于纳米-羟基磷灰石上的形态学观察

Morphology of elderly people's periodontal ligament cells attached to the three-dimensional porous nano-hydroxylapatite

目的:老年人牙周膜细胞的再生能力相对较低,选择合适的支架材料很重要。文中观察接种在三维多孔纳米-羟基磷灰石支架材料上的老年人牙周膜细胞组织形态学表现。方法:将原代培养的老年人牙周膜细胞接种于三维多孔纳米-羟基磷灰石上,在培养15 d后,采用扫描电子显微镜观察细胞的生长情况,并与青年人牙周膜细胞进行比较。结果:老年人牙周膜细胞接种到支架材料上15 d后表现为星形或多角形,有良好伸展性和对支架材料的黏附性,一定量的钙结节生成,但产生的钙结节量较青年人牙周膜细胞少。结论:老年人牙周膜细胞可以在三维多孔纳米-羟基磷灰石支架材料上良好生长。三维多孔纳米-羟基磷灰石支架材料可以作为老年人牙周组织工程治疗中的支架材料。

Objective ： Because of the lower proliferation ability of periodontal ligament cells of elderly people, how to choose suitable scaffold materials for them becomes very important. The aim of this study was to observe the morphological manifestation of the periodontal ligament cells of elderly people seeded onto the three-dimensional porous nano-hydroxylapatite scaffolds. Methods： Periodontal ligament cells from elderly people were seeded onto the three-dimensional porous nano-hydroxylapatite scaffolds, cultured for 15 days in vitro, and observed for the morphological changes by scanning electron microscopy in comparison with those of young people. Results ： Fifteen days after seeded on the three-dimensional porous nano-hydroxylapatite scaffolds, the periodontal ligament cells of the elderly people exhibited sufficient adhesive behavior, proliferated well and produced tiny calcium nodules, but the number of the nodules was smaller than that in the young people. Conclusion ： The periodontal ligament cells of eld- erly people grow well on three-dimensional porous nano-hydroxylapatite scaffolds, and can be considered as a suitable scaffold for periodontal tissue engineering in elderly people