荧光原位杂交技术检测少枝胶质瘤1p染色体的杂合性缺失

Detection of loss of heterozygosity on chromosome 1p in oligodendrogliomas by fluorescence in situ hybridization

目的观察少枝胶质瘤中1p染色体的杂和性缺失。方法使用荧光原位杂交技术对16例少枝胶质瘤标本及5例正常对照脑组织行1p染色体检测。结果在16例少枝胶质瘤中有10例有1p染色体的杂合性缺失，占62．5％，其中6例Ⅱ级肿瘤中有5例缺失，10例Ⅲ级肿瘤中有5例缺失。结论1p染色体杂合性缺失是少枝胶质瘤的重要分子生物学特征，通过荧光原位杂交技术，可对这两类少枝胶质瘤有效地加以区分，并用于进一步的临床诊断和治疗。

Objective To explore the incidence of loss of heterozygosity （LOH） on chromosome 1 p in Chinese oligodendrogliomas. Methods Sixteen specimens of oligodendrogliomas and 5 specimens of normal control cerebral tissues （gained from the decompression operation of brain trauma） were examined by fluorescence in situ hybridization （FISH）. Results Of the 16 cases of oligodendrogliomas, 10 cases （62.5%） had LOH on chromosome lp;Of the 6 cases of Ⅱ grade oligodendrogliomas,5 cases had LOH on chromosome 1p;Of the 10 cases of Ⅲ grade oligodendrogliomas,5 cases had LOH on chromosome lp. Conclusion LOH on chromosome 1 p is a most important molecular biological character of oligodendrogliomas, and oligodendrogliomas can be distinguished by FISH method effectively.