大柴胡汤对兔动脉粥样硬化血管平滑肌层肉碱棕榈酰转移酶-1表达的影响

Effect of Dachaihutang on expression of carnitine patmitoryl transferase-1 in vascular smooth muscle layer of atherosclerotic rabbits

目的:观察大柴胡汤对兔动脉粥样硬化(AS)血管平滑肌层的肉碱棕榈酰基转移酶-1(CPT-1)表达的影响,为AS治疗寻找新途径。方法:新西兰兔36只,分为正常组、模型组、辛伐他汀组和大柴胡汤组。第9,20周检测血清血脂水平,包括胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL);第25周观察升主动脉病理组织学变化;RT-PCR测定胸主动脉血管平滑肌层CPT-1 mRNA的表达。结果:大柴胡汤组与模型组相比,血清血脂水平(TC,TG,LDL)明显降低(P<0.05),病理组织学指标包括内膜(I)/中层(M)厚度比值(I/M),I+M、内膜/中层面积比值(SI/SM)明显减小(P<0.05),CPT-1 mRNA高于模型组(P<0.05);与辛伐他汀组相比,除TG值,大柴胡汤组其余指标略高于辛伐他汀组,但都无显著性差异。结论:大柴胡汤早期应用可显著降低高胆固醇诱导的兔血脂水平,抑制动脉粥样硬化的形成,并显著增加血管平滑肌层CPT-1 mRNA的表达。

Objective： To investigate the therapeutic effect of Dachaihutang on the development of atherosclerosis （AS） in rabbits and its possible mechanism by detecting the expression level of carnitine patmitoyl transferase-1 （CPT-1） in vascular smooth muscle layer of atherosclerotic rabbits, and search the new way and evidence for AS cures. Method： Thirty six male New Zealand white rabbits were divided randomly into control group, model control group, simvastatin group and Chinese traditional medicine dachaihutang group. After 9 weeks and 20 weeks of treatment, serum total cholesterol （TC） and triglyceride （TG） and low-density lipoprotein （LDL） levels were examined. At the end of 25 th weeks, histological changes in ascending aorta were studied by HE staining and histomorphometric analysis. The gene expression of CPT-1 in vascular smooth muscle layer of thoracic aorta was detected by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR）. Result： Compared with model control group, in dachaihutang group serum TC and TG and LDL levels attenuated. Pathomorphology indicated that intima and media （I＋M） became thinned, and the ratios of the thickness of intima to media（I/M） and the area of intima to media （SI/SM） were decreased （P〈0.05）. Aortic intimal proliferation in Dachaihutang group was associated with a marked increase in CPT-1 expression in vascular smooth muscle layer of thoracic aorta. Compared to simvastatin group, except TG value, other values were higher in Dachaihutang group, however, there were no significant differences between the two groups. Conclusion： These findings suggest that early treatment with Dachaihutang not only induces a significant regression of arterial lesions of high cholesterol diet rabbits, but also has a crucial inhibited genesis and development of atherosclerosis effect by up-regulating CPT-1 expression in vascular smooth muscle layer.