血管紧张素-(1-7)对大鼠肾系膜细胞TGF-β_1/Smad/CTGF通路的影响

Effect of angiotensin-(1-7) on the TGF-β1/Smad /CTGF pathway of cultured glomerular mesangial cell

目的探讨血管紧张素-(1-7)[Ang-(1-7)]对大鼠肾系膜细胞TGF-β1/Smad/CTGF通路的影响。方法采用WST法检测培养系膜细胞(GMC)增殖;RT-PCR法检测细胞内信号传递分子Smad3/7及结缔组织生长因子(CTGF)基因mRNA的表达。结果与对照组比较,Ang-(1-7)、明显抑制TGF-β1诱导的GMC增殖(P<0.05),同时,Ang-(1-7)对TGF-β1诱导的系膜细胞内Smad3、Smad7及CTGF基因mR-NA表达有明显的抑制作用(P<0.05)。结论Ang-(1-7)对TGF-β1诱导的系膜细胞增殖有抑制作用,其机制可能与抑制TGF-β1/Smad/CTGF通路有关。

[Objective] To investigate the effect of Angiotensin-（1-7） [Ang-（1-7）] on the TGF-β1/Smad/CTGF pathway of Cultured Glomerular Mesangial Cell. [Methods] Cell proliferation were evaluated by WST assay, and Smad3/7, CTGF mRNA expression in GMC were analyzed by RT-PCR. [ Results ] Compared with control groups, Ang-（1-7） obviously inhibit cultured GMC proliferation induced by TGF-β1 （P 〈0.05）;and also inhibit the mRNA expression of Smad3/7and CTGF induced by TGF-β1 in cultured GMC （P 〈0.05）. [Conclusion] Ang-（1-7） can inhibit the proliferation of GMC induced by TGF-β1 and the mechanisms may be associated with effects of suppressing TGF-β1/Smad/CTGF pathway.