L型钙通道α_(1c)蛋白在犬肺静脉前庭中表达的研究

Protein expression of the L-type Calcium channel α_(1c) subunit of pulmonary vein antrum in canines

目的研究快速起搏心房引起心房颤动(简称房颤)动物模型肺静脉前庭中L型电压依赖性钙通道(L-type voltage dependent calcium channel,LVDCC)α1c蛋白表达。方法通过建立犬房颤模型,并按是否产生房颤设立房颤组、窦性心律组和对照组,应用免疫组织化学方法检测各组肺静脉前庭中LVDCCα1c蛋白表达,用图像分析系统对组织化学抗原表达进行半定量分析。结果α1c蛋白阳性表达在发生房颤的犬肺静脉前庭组织中表达明显下降,与对照组比较差异有显著性(P<0.05)。而同样条件下,不能诱发房颤的犬肺静脉前庭组织中α1c蛋白表达与对照组相比差别无显著性(P>0.05)。结论肺静脉前庭组织中LVDCC蛋白表达的变化可能参与了心房的电重构和心房颤动的发生和维持,该通道的变化也可能是房颤难以自发终止的原因之一,也可能是房颤发生的机制之一。

[ Objective] To investigate the protein expression of L-type voltage dependent Calcium channel （LVD- CC）α1c subunit of pulmonary vein antrum in the animal model of atrial fibrillation with atrial rapid pacing. [Methods] Through setting up the animal model of atrial fibrillation in canines ,the canines were divided into three groups which were atrial fibrillation, sinus rhythm and control group respectively according to occurrencing of atrial fibrilla- tion. The expressions of LVDCC α1c protein in pulmonary vein antrum were measured by immunohistochemistry in three groups, semi-quantitative analysis of α1c protein expression was performed with pathological image analytic system. [ Results] Compared with control group, the protein expression of LVDCC α1c decreased significantly in group of atrial fibrillation, but the group of sinus rhythm had no change. [ Conclusion] The changes of protein expression of LVDCC α1c may participate in atrial eleetrial remodeling and occurrence and maintain of atrial fibrillation. The changes of LVDCC maybe one of the causes of atrial fibrillation which was hard to spontaneous termination, also maybe one of mechanisms of the atrial fibrillation.