新型单分散血清白蛋白印迹微球的制备

Preparation of monodisperse imprinting microspheres for Serum Albumin

以猪血清白蛋白(PSA)为模板,采用分级印迹方法,制备了新型单分散多孔蛋白质表面印迹微球.将PSA吸附在5μm粒径、1000孔径的球形硅胶表面及孔内后,将甲基丙烯酰胺、甲基丙烯酸为功能单体、甲叉双丙烯酰胺为交联剂的聚合物溶液,通过真空负压引入到硅胶孔内,并在室温下聚合24h.反应完成后,用3mol/LNH4HF2刻蚀硅胶,获得形状和结构与硅胶颗粒互补的PSA印迹聚合物微球.竞争吸附实验结果表明,在非模板蛋白质存在的情况下,实现印迹颗粒对模板蛋白的高选择性吸附,选择因子达到3.6,说明该印迹聚合物材料有望成为一种可以同生物抗体相媲美的新型亲和材料.

monodisperse imprinted microspheres with porcine serum albumin （PSA） as the template were prepared by hierarchical imprinting. Methacrylamide and methacrylic acid were used as functional monomers, and N, N＇-bisacrylamide, as cross-linkers. Silica （5 μm, 1000 A） was used as porogen. After polymerization, silica was etched by 3 mol/L NH4HF2 and imprinting microspheres were obtained which morphology was just complementary with silica. The binding experiment showed that MIPs exhibit high selectivity and affinity to the template protein-PSA in a competitive environment and the imprinting factor of MIPs for PSA was about 3.6. This easy preparation protocol of imprinted microspheres makes the approach a promising solution to synthesize ＂artificial antibodies＂.