液相色谱-串联质谱分析盐胁迫下植物激素的含量变化

Content change of endogenous plant hormones under salt stress by liquid chromatography-tandem mass spectrometry

建立了高效液相色谱-离子阱串联质谱高灵敏、快速测定多种内源植物激素的新方法.在最佳条件下,吲哚乙酸(IAA)、脱落酸(ABA)和赤霉酸(GA3)在5min内实现完全分离,最低检测限为8.0ng·mL–1.借助串联质谱技术,解释了三种激素的结构碎裂过程.探讨了盐胁迫下,上述内源激素含量的变化趋势.研究表明,随着NaCl浓度增大,IAA和GA3含量降低,ABA含量明显升高.初步揭示了内源激素和植物抗盐性之间的相互关系.

A quick quantitative analysis of endogenous plant hormones, such as indole-3-acetic acid （IAA）, gibberellic acid （GA3） and abscissic acid （ABA） is investigated by liquid chromatography-ion trap tandem mass spectrometry Under the optimum conditions, the above three plant hormones are separated and detected within 5 min with a detec- tion limit of 8.0 ng.mL-l. By means of MS/MS technique, the fragmentation process of these hormones is discussed in detail. The content change of endogenous plant hormones under salt stress is studied. Researches show that with the increase of NaCl concentration, levels of IAA and GA3 are diminished, while, level of ABA was upgraded obviously. The relationship between plant hormones and salt tolerance is discussed as well.