COX-2、VEGF在口腔扁平苔藓、口腔白斑及口腔鳞状细胞癌中的表达

Study on expression and relation of COX-2 &VEGF in oral lichen planus,leukoplakia and squamous cell carcinoma

目的研究环氧合酶(COX)-2、血管内皮生长因子(VEGF)在口腔扁平苔藓(OLP)、口腔白斑(OLK)及口腔鳞状细胞癌(OSCC)组织中的表达及分布,探讨其在OLP、OLK癌变机制中的作用。方法采用免疫组化法检测33例OLP患者(单纯扁平苔藓19例,伴异常增生14例)、35例OLK患者(单纯增生14例、伴异常增生21例)、31例OSCC患者及10例正常对照者口腔黏膜组织(NOM)中COX-2、VEGF的分布和表达。应用统计分析软件SPSS 13.0对结果进行统计分析。结果COX-2、VEGF在正常口腔黏膜组织无表达或低表达;在单纯扁平苔藓组均为26.32%过表达;在扁平苔藓异常增生组分别为71.43%、64.29%过表达;在白斑单纯增生组过表达情况均为21.42%;在白斑异常增生组分别为80.95%、71.43%过表达;在口腔鳞状细胞癌组分别为93.55%、90.32%过表达;COX-2、VEGF在扁平苔藓异常增生组的表达明显高于单纯扁平苔藓组(P<0.05),低于OSCC组(P<0.05),与白斑异常增生组无明显差异(P>0.05)。COX-2的表达与VEGF表达呈明显正相关(r=0.595,P<0.01)。结论COX-2、VEGF在NOM、OLP及OLK伴异常增生、OSCC的表达逐渐增高,表明COX-2及VEGF与OLP、OLK的异常增生及癌变有关。

Objective To investigate the roles of COX-2 and VEGF in the genesis of oral squamous cell carcinoma by means of evaluation of their expressions in oral lichen planus, leukoplakia and squamous cell carcinoma. Methods The expressions of COX-2 and VEGF were studied in 33 cases of oral lichen planus（OLP） ,35 cases of oral leukoplakia （OLK） , 31 cases of oral squamous cell carcinoma（OSCC） and 10 cases of normal oral mucosa by SP immunohistoehemistry. Results 1. The overexpression rates of COX-2 and VEGF in the OLP were 26. 3% ,71.43% and 64. 29% in the OLP with dysplasia, 21.42% in the OLK, 80. 95% and 71.43% in the OLk with dysplasia, 93.55% and 90. 32% in the OSCC. The expression intensity of COX-2 and VEGF in the OLP with dysplasia was higher than that in the OLP （ P 〈 0. 01） , and there were no significant differences between the OLP and OLK with dysplasia （ P 〉 0. 05）. The expression intensity of COX-2 and VEGF in the OSCC was higher than that in the OLP and the OLK with dysplasia （P 〈 0. 05 ）. Conclusion The abnormal expressions of COX-2 and VEGF are correlated with the eaneeration of OLP and OLK.