摘要
Objective:PCBP1 is a family member of heterogeneous nuclear ribonucleoproteins (hnRNPs) that belong to RNA-binding proteins and bear three KH domains. The protein plays a pivotal role in post-transcriptional regulation for RNA metabolism and RNA function in gene expression. We hypothesized and were going to identify that the regulatory function of PCBP1 is performed through different complexes of proteins that include PCBP1. Methods:To test our hypothesis,approaches of protein wal-king with a yeast two-hybrid system (Y2H),pulling down in yeasts,co-immunoprecipitation and immunofluorescent microscopy assay were employed in this study. The PCBP1 was used as the initial "walker" to search for its interaction partner(s). Results:Candidate proteins including MYL6,PECAM1,CSH1,RAB7,p57KIP2,ACTG1,RBMS1 and PSG4-like were identified with selection mediums and preceding methods. Conclusion:With these candidate protein molecules,some protein complexes associating with PCBP1 are proposed,which may help in a better understanding of physiological functions of PCBP1 and proved evidence that PCBP1 is involved in variant biological pathways.
Objective：PCBP1 is a family member of heterogeneous nuclear ribonucleoproteins （hnRNPs）
that belong to RNA-binding proteins and bear three KH domains. The protein plays a pivotal
role in post-transcriptional regulation for RNA metabolism and RNA function in gene
expression. We hypothesized and were going to identify that the regulatory function of PCBP1
is performed through different complexes of proteins that include PCBP1. Methods：To test our
hypothesis,approaches of protein wal-king with a yeast two-hybrid system （Y2H）,pulling down
in yeasts,co-immunoprecipitation and immunofluorescent microscopy assay were employed in this
study. The PCBP1 was used as the initial ＂walker＂ to search for its interaction partner（s）.
Results：Candidate proteins including MYL6,PECAM1,CSH1,RAB7,p57KIP2,ACTG1,RBMS1 and PSG4-like
were identified with selection mediums and preceding methods. Conclusion：With these candidate
protein molecules,some protein complexes associating with PCBP1 are proposed,which may help
in a better understanding of physiological functions of PCBP1 and proved evidence that PCBP1
is involved in variant biological pathways.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2009年第4期402-408,共7页
Journal of Peking University:Health Sciences
基金
Supported in part by the “985” program(985-2-035-39) of the Chinese Ministry of Education
“973” project(2007CB511902)of the Chinese Ministry of Sciences and Technology
National Nature Science Foundation(30671157)
the NewYork State Office of Mental Retardation and Developmental Disabilities(NYSOMRDD)~~
