磷霉素产生菌的定向诱变育种

Directed Mutation Breeding of Fosfomycin Producing Strain

目的:提高出发菌的磷霉素产量。方法:采用紫外(UV)+亚硝基胍(NTG)复合诱变的方法处理出发菌株(Bacillus fusi-formis),以分别含2.0和2.5mg/mL磷霉素的分离培养基来筛选UV诱变和NTG诱变后的菌株。结果:从大量突变菌中选育出一株高产、稳定的磷霉素生产菌株。当底物浓度为10mg/mL时,其磷霉素产量由1.15mg/mL提高至2.30mg/mL,转化产量提高了100%,转化率提高了10.16%。结论:采用UV+NTG诱变结合含FOM的分离平板进行定向筛选可以获得FOM高产菌株。

Objective：To increase FOM yield of Bacillus fusiforrrds. Method： UV and NTG was used as mutagen in treating FOM- producing strain. The medium containing 2.0 and 2.5mg/mL FOM was used as separation plate medium to select the mutant with UV and NTG mutation separately. Result：A high yield, stable strain was gotten, which could improve FOM production from 1.15mg/mL to 2.30mg/mL when substrate con- centration was 10mg/mL. The yield was 100% higher than the original strains and the conversion rate was 10.16% higher than the original strains. Conclusion： It＇ s an effective way to obtain lfigh yield FOM producer by using UV ＋ NTG and by using separation plate medium containing FOM.