BCL-2与P53在腭发育及腭裂形成中的作用

The effects of BCL-2 and P53 on the palatogenesis and formation of cleft palate

目的探讨BCL-2和P53在地塞米松诱导胎鼠腭裂形成中的表达及意义。方法以腹腔注射醋酸地塞米松诱导胎鼠腭裂畸形,应用免疫组织化学法和图像分析研究BCL-2和P53在不同时期实验组与对照组中腭突间充质(EPM)细胞的表达情况。结果1.对照组中BCL-2表达随着腭部发育而逐渐降低;P53表达在妊娠(gestationday,GD)13.5～15.5d中随时间变化降低,而在GD16.5增高;2.实验组中BCL-2表达GD13.5～15.5降低,GD16.5中增高;P53表达GD14.5中最高,GD15.5中最低;3.与对照组比较,实验组GD14.5～15.5中BCL-2表达明显降低(P<0.05),而P53表达明显增高(P<0.01);GD16.5中BCL-2表达明显高于对照组(P<0.01),而P53表达明显低于对照组(P<0.05)。结论地塞米松诱导的腭裂与EPM细胞凋亡水平升高有关。

Objective To investigate significance and effects of BCL-2 and P53 on development of cleft palate induced by dexamethasone. Methods The cleft palate of mice were got by injecting intraperitioneaUy with dexamethasone acetate. The different times expressions of BCL-2 and P53 in embryonic palatal mesenchyme（EPM） cell, which in the experimental groups and control group were detected by immunohistochemical method and image analysis. Results 1. In the control group, the expressions of BCL-2 gradually decreased along with the development, however the expressions of P53 gradually decreased from GD13.5 to 15.5, then increased on GD16.5.2.In the experimental groups the expressions of BCL-2 decreased from GD13.5 to 15.5, then increased on GD16.5; the expressions of P53 were the highest on GD14.5 and the lowest on GD15.5.3. Compared with control group, the expression of BCL-2 significantly decreased （P 〈 0.05）; While the expressions of P53 increased significantly （P 〈 0.01）, which happened in the experimental groups from GD13.5 to 15.5; On GD16.5 the expression of BCL-2 remained significantly higher than control group（ P 〈 0.01 ） ,however the expressions of P53 remained significantly lower than control group（ P 〈 0.05）. Conclusion There is a relationship between increasing level of apoptosis and development of cleft palates induced by dexamethasone.