用彗星试验研究氯化消毒中水对小鼠脾淋巴细胞DNA的损伤作用

DNA damage by chlorinated disinfection during water reuse in the lymphocytes from the spleen of mice assessed by the comet assay

目的了解氯化消毒中水对小鼠脾淋巴细胞DNA分子的损伤作用。方法采用单细胞凝胶电泳试验(彗星试验)分别对前后的中水水样进行检测。结果消毒后水样可引起小鼠脾淋巴细胞DNA分子断裂增加,而且在相同剂量水平上,消毒后水样对小鼠脾淋巴细胞DNA分子的损伤作用明显高于消毒前水样(1μl/ml组消毒前水样淋巴细胞头部DNA含量为7.77%±15.78%,消毒后水样淋巴细胞头部DNA含量为0.95%±2.78%)。结论氯化消毒过程可造成中水的遗传毒性显著增加,可以采用单细胞凝胶电泳试验对这种遗传毒性进行检测。

Objective To investigate the changes of the DNA of the lymphocytes from the spleen of mice after the treatments of water samples collected before and after the chlorinated disinfection in the water reuse procedure. Methods The Comet Assay was occupied. Untreated control, solvent control and positive control were included as needed. Results All of the results of the two water samples in the three different levels were positive, the sample which collected after disinfection shown greater damage effects on the DNA of the lymphocytes from the spleen of mice than the sample collected before disinfection. Conclusion These results indicated that the chlorinated disinfection process among the water reuse procedure could increase the genotoxicity of the water, And the comet assay could be used to assess the genotoxicity of the reused water.