宫颈癌组织中抑癌基因Syk启动子甲基化的研究

METHYLATION OF Syk GENE PROMOTER IN CERVICAL CANCER

目的探讨宫颈癌组织中脾酪氨酸激酶(Syk)基因启动子甲基化程度及其临床意义。方法采用甲基化特异性PCR(MSP)方法检测60例宫颈癌、50例宫颈上皮内瘤变(CIN)和20例正常宫颈组织中Syk基因启动子甲基化情况。结果宫颈癌组织中Syk基因启动子甲基化率明显高于CIN组织及正常宫颈组织(χ2=17.13、19.71,P<0.05);Syk基因甲基化程度在宫颈癌组织中的表达与肿瘤淋巴结转移有明显相关性(χ2=10.22,P<0.01),但与病人年龄、肿瘤大小、临床分期、组织学类型及病理类型无明显相关性(P>0.05)。结论Syk基因启动子甲基化可能与宫颈癌发生及其淋巴转移有关。

Objective To explore the methylation of spleen tyrosine kinase Syk gene promoter and its clinical significance in cervical cancer. Methods Using methylation specific PCR technique, specimens from 60 cervical cancer patients, 50 cervical intraepithelial neoplasia （CIN） patients, and 20 normal cervical tissue were detected for methylation of Syk promoter. Results The methylation rate of Syk gene in cervical cancer tissue was significantly higher than that in CIN and normal cervical tissue （Х^2=17.13,19.71;P〈0.05）. The methylation of Syk gene in the cancer tissue was markedly correlated with lymph node metastasis （Х^2=10. 22,P〈0.01）, but not associated with patients＇ age, size of tumor, clinical stage, types of histology and pathology （P〉0.05）. Conclusion Methylation of Syk gene promoter may be correlated with oncogenesis and lymph node metastasis of cancer of the cervix.