摘要
以早抽薹品种CT07和晚抽薹品种T0601为亲本构建F2分离群体,采用分离集团混合分析法(Bulkedsegregant analysis,BSA)筛选与菜薹抽薹基因连锁的分子标记。用45条ISSR(Inter-simple sequence repeat,简单重复序列间扩增)引物和20对SRAP(Sequence related amplified polymorphism,序列相关扩增多态性)引物组合进行PCR扩增筛选,其中UBC834、UBC876、E13M4和E5M7对亲本和DNA池的扩增图谱表明,4个引物的差异条带均出现在早抽薹品种CT07和早现蕾(抽薹)池中。经F2代群体单株验证,4个标记均与早抽薹基因相连锁,且标记E13M4最近,距离为16.8 cM,这为菜薹抽薹基因的定位和分子标记辅助育种奠定了基础。
The F2 population derived from a cross between CT07 （early bolting） and T0601 （late bolting） was used to identify bolting trait using ISSR and SRAP makers by BSA analysis. 45 ISSR primers and 20 SRAP primers combinations were used to screen polymorphism in parents and DNA pools. PCR pattern in parents and DNA pools showed different fragment appeared in CT07 and early bolting pools by UBC834, UBC876, E13M4 and ESM7 primers. Bolting trait was linked to four makers and the closest marker to bolting gene was E13M4 and the distance between them was 16. 8 cM through F2 generation individuals identification. The four makers in the study could be used to maker assistant breeding.
出处
《江苏农业学报》
CSCD
北大核心
2009年第4期829-833,共5页
Jiangsu Journal of Agricultural Sciences
基金
江苏省农业高技术项目(BG2005312)
