万寿菊花超临界萃取物的抗氧化活性

Antioxidant Activity of Supercritical CO_2 Extracts from Marigold Flower

采用超临界CO2萃取技术辅以乙醇为夹带剂,制备万寿菊花超临界萃取物(Supercritical CO2 extracts of marigold flower,SEMF)。从抗脂质过氧化能力、还原能力及对羟基自由基(.OH)和超氧阴离子自由基(O2-.)的清除能力方面,综合考察SEMF的体外抗氧化活性。结果表明:SEMF对脂质过氧化有较强的抑制能力,并具有较强的还原能力,其清除.OH和O2-.的EC50分别为0.00083 mg/ml、0.00074 mg/ml(以叶黄素计)。SEMF在这些体系中均表现出较强的抗氧化能力,表明SEMF是一种优良的天然抗氧化剂和自由基清除剂。

The extracts from marigold flower were obtained by supercritical CO2 extraction with ethanol modifier. Its antioxidant activities were evaluated by antilipid peroxidation, reducing power, as well as hydroxyl radical （·OH） and superoxide ion radical （O2^-·） scavenging. The supercritical CO2 extracts from marigold flower exhibited strong antioxidant activities to peroxidation and reducing power. EC50 expressed by the supercritical CO2 extracts reflecting scavenging effects on · OH and O2^- · were 0. 000 83 mg/ml,0. 000 74 mg/ml,respectively （caculated in lutein content）. The overall results suggested that the supercritical CO2 extracts would be an excellent natural antioxidant and free radical scavenger.