摘要
目的 观察荷叶黄酮对大鼠心肌缺血再灌注损伤的保护作用，并探讨其可能的作用机制。方法 将4月龄雄性Wistar大鼠50只，随机分为对照组（10只）、模型组（20只）和治疗组（20只）。建立大鼠心肌缺血再灌注损伤模型，模型组和治疗组大鼠于冠状动脉结扎和再灌注前1min分别由左心腔缓慢注射生理盐水、荷叶黄酮（60mg／kg）。2h后处死动物，应用苏木精-伊红染色观察组织形态学的改变，应用免疫组织化学方法测定心肌组织中Bax蛋白、Bcl-2蛋白及Caspase-3的表达。结果 治疗组细胞水肿较模型组明显减轻。与模型组比较，治疗组Bax及Caspase-3蛋白的表达均明显降低，而Bcl-2蛋白的表达明显升高（F=3．33～4．17，q=3．34～8．03，P〈0．05）。结论 荷叶黄酮对大鼠心肌缺血再灌注损伤具有保护作用，其机制可能与上调Bcl-2、下调Bax和Caspase-3的表达，减少细胞凋亡有关。
Objective To investigate the protective effect of Nelumbo Flavone on myocardium ischemia-reperfusion injury in rat and probe into the mechanism of protection. Methods Fifty 4-month-old male SD rats were randomly divided into three groups： control group （10 rats）, model group （20 rats）, and treatment group （20 rats）. A rat model of myocardium ischemiareperfusion injury was established. Rats in the model and treatment groups received intraventricular injection of normal saline and Nelumbo Flavone （60 mg/kg）, respectively, one minute before coronary artery ligation and reperfusion. The animals were sacrificed two hours later. Eosin-hematoxylin （HE） staining was performed for morphology observation and immunohistochemistry staining was used to study the expressions of Bax, Bcl-2 and Caspase-3 proteins in myocardium. Results Compared with the model group, there was less myocardium edema in the treatment group. The expressions of Bax and Caspase-3 proteins were significantly lower and the expression of Bcl-2 was significantly higher in the treatment group （F= 3.33 - 4. 17; q = 3.34 - 8.03;P〈0.05）. Conclusion Nelumbo Flavone shows protective effects on rat myocardium ischemia-reperfusion injury. The mechanism of protection might be related with up-regulation of Bcl-2, down-regulation of Bax, inhibition of Caspase-3 and reduction of cell apoptosis.
出处
《齐鲁医学杂志》
2009年第6期515-516,518,共3页
Medical Journal of Qilu
