高效液相色谱法测定杨梅中有机酸

DETERMINATION OF ORGANIC ACID IN REDBAYBERRY BY HPLC

采用高效液相色谱法测定杨梅中的有机酸,流动相为0.02 mol/L的NaH2PO4,检测波长为210 nm。柠檬酸、苹果酸、酒石酸、乳酸的检出限分别为5、3、1、10 mg/L,测定结果的相对标准偏差分别为0.2%、0.3%、0.1%、2.4%,线性范围分别为0.01-0.7 g/L、0.05-0.7 g/L、0.1-0.7 g/L、0.1-0.7 g/L,线性相关系数分别为0.9999、0.9997、0.9911、0.9944。实验结果表明,杨梅中含有柠檬酸、苹果酸、乳酸、酒石酸、草酸,其中以柠檬酸含量最高。

Organic acid in redbayberry was determined by high performance liquid chromatography.The mobile phase was 0.02 mol / L NaH2PO4,and detection dewavelength was 210 nm.The detection limits of citric acid,malic acid,lactic acid and tartaric acid were 5,3,1,10 mg/L respectively.The relative standard deviations were 0.2%,0.3%,0.1%,2.4% respectively,and the linear ranges were 0.01-0.7 g/L,0.05-0.7 g/L,0.1-0.7 g/L,0.1-0.7 g/L with the linear correlation coefficients of 0.999 9,0.999 7,0.991 1,0.994 4 respectively. The result showed that redbayberry contained citric acid, malic acid, lactic acid, tartaric acid and oxalic acid. The content of citric in redbayberry acid was the highest.