傅里叶变换红外光谱法研究核辐照对螺旋藻粉成分的影响

Study on Effect of Irradiation on Components in Spirulina Powder by Fourier Transform Infrared(FTIR) Spectroscopy

采用红外光谱法对经不同剂量核辐照的螺旋藻粉进行了对比研究,比较了它们的红外光谱的异同。螺旋藻粉的红外光谱主要由蛋白吸收带及碳水化合物的吸收带组成,一定辐照剂量处理的螺旋藻粉成分的化学结构并未发生变化,部分成分含量发生了变化。表明辐照可杀灭螺旋藻粉中污染微生物和各种水生寄生虫卵,同时利用红外光谱法可以快速检测螺旋藻粉样品是否经过辐照处理。随着辐照剂量的增加样品某些种类的蛋白质含量随之减少了。这与文献[1]研究得到的结果是一致。螺旋藻粉的红外光谱的特征峰(1654、1452及1240cm-1)与啤酒酵母的蛋白质红外光谱的特征峰位相同及峰形非常相似,表明螺旋藻粉和啤酒酵母中的蛋白质的化学结构可能很相似。

The different irradiation dose s on spirulina powder were studied by fourier transform infrared（FTIR） spectroscopy.The Infrared spectra of spirulina powder is mainly made up of protein absorb belt and carbohydrates absorb belt.Dealing with spirulina powder by definite irradiation dose,component s chemistry structure don t change,the content of part component change.The irradiation is advisable to kill polluted microorganism of spirulina powder and all kinds of aquatic parasite ovum.At the same time,infrared spectra can quickly examine spirulina powder sample whether or not dealing with irradiation. As the irradiation dose increases,the content of the sample＇s some kind of protein reduces. This is the same to the result of the document 1. The characteristic peak of infrared spectra of spirulina powder is the same to the one of infrared spectra of beer yeast protein,Their peak shapes are similar very much,obviously, the chemistry structure of spirulina powder and the protein of beer yeast may be similar.