食管癌患者亚甲基四氢叶酸还原酶基因多态性与饮酒的交互作用研究

Interaction study between MTHFR C677T mutation and alcohol taking on esophageal cancer

目的探讨亚甲基四氢叶酸还原酶（MTHFR）基因C677T及A1298C多态性与饮酒间交互作用对食管癌发病的意义。方法以新诊断的食管癌患者为研究对象,采用病例-对照研究设计,用聚合酶链反应-限制性片段长度多态性分析法（PCR-RFLP）检测MTHFR基因C677T及A1298C多态基因型,运用相乘模型分析MTHFR基因C677T及A1298C多态性与饮酒交互作用对食管癌发病的影响。结果MTHFR基因C677T多态基因型与饮酒指数间存在次相乘模型的交互作用,OR为10.892（2.461-65.927）;MTHFR基因A1298C多态基因型与饮酒指数间存在超相乘模型的交互作用,OR为8.339（1.131-61.459）。结论MTHFR基因C677T及A1298C多态基因型可增加过量饮酒者食管癌的患病危险性。

Objective To explore the effect of 5,10-methylenetetrahydrofolate reductase （MTHFR） mutation and alcohol taking on esophageal cancer （EC）. Methods Case-control design was used and newly diagnosed EC patients were recruited as subjects. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was performed to detect MTHFR gene mutation. The interaction between MTHFR gene mutation and alcohol taking was evaluated by multiple Logistic regression analysis. Results There was synergism of multiplieative interaction between MTHFR gene mutation and alcohol taking. The OReg of C677T and alcohol taking was 10. 892 （2. 461-65. 927 ）, and the OReg of A1298C and alcohol taking was 8. 339 （ 1. 131-61. 459）. Conclusion MTHFR gene mutation can increase risks of EC in subjects with alcohol drinking.