计算分光光度法应用于牡丹皮中丹皮酚的测定考察

Investigation on Computative Spectrophotometry Employed in the Assay of Peaonol in Cortex Moutan

目的：考察计算分光光度法在中药分析中应用的可行性。方法：用三波长法和双波长系数倍率法测定了１０批牡丹皮样品中丹皮酚的含量，并与药典法进行比较。结果：由于不同批次药材的紫外光谱的差异，导致计算分光光度法测定的相对误差有时可达１０％～３０％。

Objective:To investigate the easibility of employed computative spectrophotometry in the assay of traditional Chinese medicines. Method: Make contents of peaonol in 10 samples of Cortex Moutan were determined by threewavelength spectrophotometry and dual wavelength Kratio spectrophotometry,and then compared with the method in Chinese Pharmacopeia.Result:The differences of the UV spectrum of different lots of samples bring about relative error.sometimes up to 10%～30% for computative spectrophotometry.Conclusion:Computative spectrophotometry should be used carefully in assaying traditional Chinese medicines. 