病毒性脑炎患儿血清及脑脊液中基质金属蛋白酶-9及其抑制剂的变化及临床意义

Clinical significance and changes of cerebrospinal fluid and serum matrix metalloproteinase-9 and matrix metalloproteinase inhibitors in children with viral encephalitis

目的探讨病毒性脑炎患儿血清和脑脊液中基质金属蛋白酶-9(MMP-9)和基质金属蛋白酶组织抑制剂-1(TIMP-1)水平的变化及临床意义。方法采用酶联免疫吸附试验(ELISA)检测48例病毒性脑炎患儿和25例正常患儿血清和脑脊液中MMP-9、TIMP-1水平。结果病毒性脑炎患儿轻型组和重型组血清和脑脊液中基质金属蛋白酶-1和基质金属蛋白酶组织因子-1及其二者比值均明显高于对照组(P<0.05或0.01),且重型组较轻型组明显增高(P<0.05)。结论检测病毒性脑炎患儿血清和脑脊液中MMP-9、TIMP-1水平及MMP-9/TIMP-1比例对指导临床诊断和治疗具有一定的意义。

Objective To discuss the clinical significance and changes of cerebrospinal fluid and serum matrix metalloproteinase-9（MMP-9） and tissue inhibtor of metalloproteinase-1（TIMP-1） in children with viral encephalitis. Methods Enzymelinked immunosorbent assay （ELISA） was adopted for detecting serum and eerebrospinal fluid of MMP-9, TIMP-1 level in 48 cases of children with viral encephalitis and 25 cases of healthy children. Results Serum and cerebrospinal fluid matrix metalloproteinase -1 and tissue inhibitor of metalloproteinase-1 and the ratio of MMP-9/TIMP-1 in mitis group and gravis type group of children with viral encephalitis were significantly higher than that in healthy children group （P〈0.05 or 0. 01） ,and the gravis type group was significantly higher than that in mitis group（P〈0.05）. Conclusion Detection of serum and cerebrospinal fluid of MMP-9, TIMP-1 level and the ratio of MMP-9/TIMP-1 in children with viral encephalitis had a certain significance for guidance on the clinical diagnosis and treatment.