应用实验性肝转移模型研究肝癌术后转移因素

FACTORS INFLUENCING POSTOPERATIVE METASTASIS OF MOUSE HEPATOMA

目的探讨肝癌术后转移的影响因素。方法６１５小鼠脾包膜下接种肝癌Ｈ２２腹水瘤细胞，在脾接种前后的不同时间进行部分肝切除或假手术，并比较不同程度手术创伤对肿瘤转移的影响。结果假手术组和部分肝切除组与单纯脾接种组比较均对肿瘤转移起促进作用，其中又以部分肝切除促肿瘤转移效应最显著，且１／３肝切除重于１／４肝切除。手术促肿瘤转移的时间以术后３天内最显著。结论手术创伤尤其是部分肝切除对肝癌转移起促进作用，并与转移程度呈正相关。

Objective:To study the influence of the surgical trauma on hepatocarcinoma metastasis.Methods:The 615 mouse hepatoma H22 ascites cells were inoculated into 615 mouse spleen subserous layer in simulating the primary lesion. After partial hepatectomy or sham operation ,the metastatic foci on liver surface were recorded.Results:In compare with simple spleen inoculation of H22 cells,the most significant metastasisenhancing effect was seen in partial hepatectomy group and sham operation group 1/3 hepatectomy was more remarkable than 1/4 hepatectomy. This enhanced effect was found within the first three days after surgery.Conclusion:Surgical trauma is involved in enhanced metastasis, and there is a significant correlation between the degree of trauma and the stronger metastasisenhancing effect.