自体骨膜细胞移植治疗骨不连的实验研究

AUTOGENEIC PERIOSTEAL CELL TRANSPLANTATION FOR THE TREATMENT OF NONUNION OF BONE IN RABBITS

目的：研究自体骨膜细胞移植能否促进骨修复，探讨治疗骨不连的新方法。方法：用健康新西兰兔１０只，切取胫骨前内侧骨膜，进行骨膜细胞培养，然后将培养的单层骨膜细胞收集后移植至供体动物一侧的桡骨缺损内，对侧桡骨缺损做对照。术后２、４、６和８周进行Ｘ线平片和组织病理学检查。结果：大多数实验侧于术后６～８周发生骨性愈合；而对照侧均未发生骨性愈合。结论：自体骨膜细胞移植具有促进骨修复的作用。

Objective:To evaluate the effect of autogeneic periosteal cell transplantation on the repair of nonunion of bonesMethods:Ten New Zealand rabbits were usedThe anteromedial periostea of the tibiae were excised and culturedThe monolayer culture of periosteal cells were collected and transplanted into the radial bone defect on one side in each rabbit,and the bone defect on the contrtalateral side without periosteal cell transplantation served as the controlRadiography and histologic examination were made after the cell transplantationResults:Bone union occurred in 6 to 8 weeks on most of the test sides except in two rabbitsIn the control sides,none of the bone defects healedConclusion:This indicates that autogeneic grafting of periosteal cells could improve the repair of nonunion of bone