山楂总黄酮对血瘀合并脑缺血-再灌注小鼠的影响(一)

Effect of Halothorn Flavonoids on blood stasis complicated with cerebral ischemic reperfusion of mice(Ⅰ)

目的:观察山楂总黄酮对小鼠血瘀性脑缺血-再灌注模型全血黏度、脑含水量及脑组织损伤的影响。方法:用地塞米松复制小鼠血瘀模型;结扎双侧颈总动脉30min,再灌20min复制脑缺血-再灌注模型;通过测定该模型小鼠全血黏度、脑水肿、Ca2+和观察脑组织病理学的情况,探讨山楂总黄酮对小鼠血瘀性脑缺血-再灌注模型血液流变性、脑含水量、Ca2+和脑组织的影响。结果:山楂总黄酮能明显改善血瘀性脑缺血-再灌注小鼠的全血黏度(P<0.05),能显著或明显减轻脑组织Ca2+的超载程度(P<0.01或P<0.05),明显减轻脑水肿程度(P<0.05),对脑神经细胞和胶质细胞损伤有明显的拮抗作用。结论:山楂总黄酮能改善小鼠血瘀性脑缺血-再灌注模型血液流变性,预防脑水肿的发生和Ca2+超载,改善脑组织的病理情况,具有保护脑缺血-再灌注损伤的作用。

Objective： To explore the effect of Halothorn Flavonoids （HFs） on cerebral energy metabolism and cerebral tissue of the blood stasis complicated with cerebral ischemic reperfusion model mice. Methods： The blood stasis complicated with cerebral ischemic reperfusion mice models were established by ligating common carotid artery for 30 min and opening 20 rain in the blood stasis mice models. The effect of HFs on blood viscosity and cerebra[ tissue of the blood stasis complicated with cerebral ischemic repeffusion model mice was judged by measuring the indexes such as Ca^2＋ in cerebral tissue and by analyzing the degree of cerebral edema and the histopatholgical changes of the model mice. Results： HFs obviously improved the blood viscosity of model mice （P〈O.05）, obviously reduced the Ca^2＋ overload （P〈0.01 or P〈0.05）, significantly decreased the degree of cerebral edema （P〈0.05）, significantly counteracted the neural cells and colloid cells. Conclusion： HFs can obviously ameliorate the degree of cerebral edema, the neural cells and colloid cells and the blood viscosity of the blood stasis combined with cerebral ischemic reperfusion model mice.