玻璃体中碱性成纤维细胞生长因子的定量

QUANTITATIVE STUDY OF bFGF IN VITREOUS OF PVR

目的：定量研究增殖性玻璃体视网膜病变（ＰＶＲ）患者玻璃体中碱性成纤维细胞生长因子（ｂＦＧＦ）的含量，阐明ｂＦＧＦ在ＰＶＲ中的作用和影响。方法：双夹心酶联免疫吸附技术测定正常对照组２０只眼、ＰＶＲＣ组３５只眼、ＰＶＲＤ组２６只眼和单纯玻璃体出血组２５只眼患者玻璃体内ｂＦＧＦ含量，以及ＰＶＲＤ病人（１５例）血清内ｂＦＧＦ含量。结果：①玻璃体内ｂＦＧＦ含量：对照组ｍｅｄｉａｎ５．２０ｎｇ／Ｌ，ｑｕａｒｔｉｌｅ１５．４７ｎｇ／Ｌ；ＰＶＲＣ组ｍｅｄｉａｎ３．１２ｎｇ／Ｌ，ｑｕａｒｔｉｌｅ１０．４８ｎｇ／Ｌ；ＰＶＲＤ组ｍｅｄｉａｎ４６．５６ｎｇ／Ｌ，ｑｕａｒｔｉｌｅ１１３．９６ｎｇ／Ｌ；单纯玻璃体出血组ｍｅｄｉａｎ１．４０ｎｇ／Ｌ，ｑｕａｒｔｉｌｅ６．２５ｎｇ／Ｌ；ＰＶＲＤ组玻璃体内ｂＦＧＦ含量明显高于对照组，也高于ＰＶＲＣ组和单纯玻璃体出血组（各Ｐ＜０．０１）；②ＰＶＲＤ组血清内ｂＦＧＦ含量：（１８．３３±３．３９）ｎｇ／Ｌ；ＰＶＲＤ组玻璃体内ｂＦＧＦ含量明显高于其血清含量，Ｐ＜０．０１；③玻璃体内ｂＦＧＦ含量与裂孔面积的关系：在ＰＶＲＤ组，裂孔大于１５ｍｍ×１５ｍｍ组ｂＦＧＦ含量较高（Ｐ＜０?

Objective: Quantitative study of basic fibroblast growth factor (bFGF) in vitreous of proliferative vitreoretinopathy (PVR), to state the result and influence of bFGF in PVR. Method:The high sensitive sandwich enzyme immunoassay technique (ELISA) was used to measure the level of bFGF in vitreous of normal group 20 eyes, PVRC group 35 eyes, PVRD gruop 26 eyes, vitreous hemorrhage group 25 eyes (no vascular proliferative retinopathy) and in serum of PVRD patients (15 cases). Results:①The bFGF level in vitreous: normal group median 5.20ng/L, quartile 15.47ng/L; PVRC group median 3.12ng/L, quartile 10.48ng/L; PVRD group median 46.56ng/L, quartile 113.96ng/L; and vitreous hemorrhage group median 1.40ng/L, quartile 6.25ng/L. The bFGF level in PVRD group was significantly higher than that in normal group, PVRC group and vitreous hemorrhage group (P<0.01). ② bFGF level in vitreous and serum of PVRD group: The bFGF level of serum was mean (18.33±3.39)ng/L; the bFGF level of vitreous in PVRD was significantly higher than that in serum (P<0.01). ③ bFGF level in vitreous and PVR risk factor:bFGF level was significantly higher in bigger retinal break group in PVRD group. Conclusion:The result implied that bFGF play an important role in PVR.