酶联免疫吸附检测鼻咽癌方法的建立

THE ESTABLISHMENT OF ENZYME- LINKED IMMUNOSORBENT ASSAY(ELISA) FOR THE DETECTION OF NASOPHARYNGEAL-CARCINOMA(NPC)

目的：探讨检测鼻咽癌的酶联免疫吸附（ＥＬＩＳＡ）方法。方法：以正丁酸和巴豆油诱导Ｒａｊｉ细胞表达的ＥＢ病毒早期蛋白为抗原，用ＥＬＩＳＡ方法检测血清中抗ＥＢ病毒相关早期蛋白的ＩｇＧ抗体水平（吸光度Ａ４９０）。其中鼻咽癌４５４例，肝癌４０例，肺癌２３例，肠癌２０例，乳腺癌２３例，卵巢癌１６例，头颈肿瘤１５例和健康人５２４例。结果：在所有被检血清中鼻咽癌血清Ａ值最高（０．３３３±０．１００），（Ｐ＜０．００１）。以正常人９５％特异性Ａ４９０＜０．１８为界，ＥＬＩＳＡ方法诊断鼻咽癌的敏感性为８９％（４０５／４５４）特异性为９４％（４９３／５２４）。结论：用来自Ｒａｊｉ细胞内的ＥＢ病毒早期蛋白为抗原建立的ＥＬＩＳＡ方法可以应用于鼻咽癌的血清学诊断。

Objective:To establish ELISA for the detection of NPC.Methods:With EB virus early proteins antigen expressed in Raji cells induced by croton oil and n-butyric acid as test antigen (Raji EA+) in ELISA, sera collected from NPC (454 cases), liver cancer (40 cases), lung cancer (23 cases) colorectal cancer (20 cases), breast cancer (23 cases) ovarian cancer (16 cases), carcinomas of the head and neck (15 cases) and healthy individuals (524 cases) were tested for the levels (A490) of IgG antibody to Raji EA+ antigen by ELISA. Results:The level (A490) of IgG antibody to Raji EA+ antigen in NPC sera group was 0.333±0.100(±s), the highest one above the 8 sera groups tested (P<0.001). Using A490≥0.18 as NPC positive threshold, the diagnostic specificity and sensitivity to NPC were 94%(493/524) and 89%(405/454). Conclusion:The EB virus early protein antigens from induced Raji cells could be used in ELISA for the detection of NPC serologically.