PCR检测白血病、再生障碍性贫血患者3种疱疹病毒

THREE HERPES VIRUSES IN PATIENTS WITH ACUTE LEUKEMIA AND APLASTIC ANEMIA

目的：观察白血病、再生障碍性贫血患者治疗前后巨细胞病毒（ＣＭＶ）、单纯疱疹病毒（ＨＳＶ）、爱博斯坦巴尔病毒（ＥＢＶ）的感染状况。方法：用ＰＣＲ法检测急性白血病患者骨髓单个核细胞内ＣＭＶＤＮＡ、ＨＳＶＤＮＡ、ＥＢＶＤＮＡ，以及再生障碍性贫血患者血浆中ＣＭＶＤＮＡ感染情况。结果：急性白血病患者骨髓单个核细胞内ＣＭＶＤＮＡ的检出率高于血浆的检出率；初治白血病患者骨髓单个核细胞内及初治再生障碍性贫血患者的血浆ＣＭＶＤＮＡ阳性率分别为７３％和５４％，明显高于正常人（Ｐ＜０．０５）；经治疗半年后未能缓解的急性白血病患者骨髓单个核细胞内３种疱疹病毒ＤＮＡ检出率高于完全缓解和部分缓解的急性白血病患者；病情无改善或恶化的再生障碍性贫血患者血浆ＣＭＶＤＮＡ检出率亦高于病情有好转的患者。结论：初治白血病患者和初治再生障碍性贫血患者血浆ＣＭＶＤＮＡ阳性率高于正常人，治疗后３种疱疹病毒ＤＮＡ的检出率在未缓解的白血病患者中较高，ＣＭＶＤＮＡ检出率在治疗效果差的再生障碍性贫血患者中较高。

Objects: To observete the infection of CMV、HSV and EBV before and after treatment in patients with leukemia and aplastic anemia. Methods: We detected CMVDNA、HSVDNA、EBVDNA in mononuclear cells of bone marrow from acute leukemic patients and CMVDNA in plasma of aplastic anemic patients. Results:The positive rate of CMVDNA with acute leukemic patients in mononuclear cells was higher than the that in plasma, and the positive rates of CMVDNA in mononuclear cells of leukemic patients and in plasma of aplastic anemic patients at the beginning of therapy were 72.9% and 54.4%, respectively, which were higher than those of normal controls (P<0.05). The positive rates of the three herpes viruses DNA in mononuclear cells of acute leukemic patients in nonregression group after six months of treatment were all higher than the ones of complete and partial regression patients. The positive rate of CMVDNA in plasma of the aplastic anemic patients without improvement or worsening condition was higher than the rate of those patients with improval. Consults:The positive rate of CMVDNA in untreated leukemic and aplastic anemic patients is highter than one of normal contol. The positive rates of three herpes viruses are high in nonremission patients after treatment. The positive rate of CMVDNA is high in aplastic anemic patients who have poor treatment result.