豚鼠噪声暴露后畸变产物耳声发射与外毛细胞的改变

Changes in distortion product otoacoustic emissions and hair cells after noise exposure in guinea pigs

为观察豚鼠噪声暴露后畸变产物耳声发射（DPOAE）与内耳毛细胞的改变，将16只健康豚鼠分为3组，正常对照组3只，噪声后即刻组3只，7d组1o只。暴露于115dB模拟潜艇机舱噪声中4h，暴露后即刻及7d检测DPOAE听力图及I／O函数曲线，光镜及扫描电镜观察耳蜗毛细胞的改变。暴露即刻组DPOAE振幅消失（P＜0．01），7d后又恢复至暴震前的基线水平（P＞0.05）。光镜及扫描电镜显示耳蜗2、3、4kHZ区域外毛细胞好纤毛排列紊乱及部分消失。提示噪声后外毛细胞静纤毛轻度损伤时所保留的正常外毛细胞可调整及代偿其功能，故DPOAE相值可为正常。

In order to observe the changes in distortion product otoacoustic emissions(DPOAE) and hair cells after noise exposure,sixteen healthy gUinea pigs were used. The animalswere divided into 3 groups- Group 1 was norrnal animals. Group 2 and 3 were animal right and 7days after exposed to 115 dB SPL simulated submarine engine room noise for 4 hours,respectively-The DPOAE audiogram and I/O function were measured before and after exposure' The changes ofhair cells were observed by light microscope and scanning electron ndcroscoPe. The amplitudes ofDPOAE disappeared right after noiSe exposure(P <0. 01) and recovered to normal at 7 days afterexposure (P >0. 05). The light microscopy and scanning electron microscopy showed the stereoci-lia of the outer hair cells were disarrangement and some disappeared dt 2,3,4 kHz regions of thecochlea- The results indicated that the normal outer cells can regUlate and compensate the functionof some damaged outer hair cells which result in a normal DroAE in mild damaged cochlea.