吡格列酮对瘦素诱导的血管平滑肌细胞PCNA及瘦素受体表达的影响

Effects of pioglitazone on leptin-induced proliferating cell nuclear antigen and leptin receptor expression of vascular smooth muscle cells

观察吡格列酮对瘦素诱导的血管平滑肌细胞（VSMCs）增殖的干预作用，分别检测VSMCs中增殖细胞核抗原（PCNA）和瘦素受体（OB-R）mRNA和蛋白表达水平的变化。结果表明吡格列酮可抑制瘦素刺激的VSMCs增殖以及PCNA和OB—R的表达，抑制效应的最佳浓度为100μmol／L（P〈0．01）。

The effects of pioglitazone on leptin-induced proliferation of vascular smooth muscle cells （ VSMCs）, proliferating nuclear antigen （PCNA） and leptin receptor （OB-R） mRNA and protein expressions were measured. The results showed that pioglitazone inhibied leptin-induced VSMCs proliferation as well as expression of PCNA and OB-R at both mRNA and protein levels. The maximum effect was at 100 μmol/L （ P〈0. 01 ）.