邻苯二甲酸二(2-乙基己基)酯致胎鼠隐睾发生过程中胰岛素样因子3的表达分析

Effect of Di-(2-ethylhexyl)Phthalate on Insulin Like Factor 3 Expression in Fetal Mice

目的:探讨环境内分泌干扰物邻苯二甲酸二(2-乙基已基)酯[Di-(2-ethylhexyl)phthalate,DEHP]诱发胎鼠隐睾的分子机制。方法:妊娠昆明小鼠随机分为3组,每组15只:DEHP实验组(A组)、玉米油(溶剂)对照组(B组)和正常对照组(C组)。A组和B组自妊娠12.5￣18.5d分别持续经口每天给予DEHP(500mg/kg)或玉米油,C组不予灌药。结果:B组和C组睾丸组织中胰岛素样因子3(INSL3)mRNA和蛋白质的表达均无差异(P>0.05);A组INSL3mRNA的表达量约为B或C组的1/3,INSL3蛋白质的表达量约为B或C组的1/4,与B或C组比其差异显著(P<0.05)。A组睾丸引带发育不良,睾丸位置异常。结论:环境内分泌干扰物DEHP下调睾丸间质细胞INSL3基因的表达,影响睾丸引带发育,可能是DEHP诱发隐睾的分子机制之一。

Objective：To explore the molicular mechanism of cryptorchidism induced by Di-（2-ethylhexyl）phthalate（DEHP）in mice.Methods：Kunming mice were divided into 3 groups randomly with 15 in each group：DEHP-exposed group（group A）,corn oil（menstrum）group（group B）and normal control group（group C）.Group A or group B was administered respectively with DEHP（500 mg/kg）or corn oil to the pregnant mice from gestation day 12.5（GD12.5）to 18.5,and group C with nothing.Results：DEHP altered gubernacular development,disrupted testis decent,and induce cryptorchidism.MRNA and protein expression of INSL3 between groups B and C were not significant（P0.05）.INSL3 mRNA expression in group A was about one-third of that in group C,which was significantly decreased（P0.05）.Similarly,INSL3 protein expression in DEHP-exposed group was about quarter of that in group A,which was also significantly decreased（P0.05）.In group C,the development of gubernaculum was not well and the site of testis was abnormal.Conclusion：DEHP as one of environmental endocrine disruptors,can down-regulate INSL3 gene expression and interfere the gubernaculum development.It may be one of possible mechanisms that DEHP cause cryptorchidism.