卵巢浆液性乳头状囊腺癌中PTEN、survivin和MMP-2的表达及临床意义

The Expression and Its Clinical Significance of PTEN,Survivin,and MMP-2 in Ovarian Serous Adenocarcinoma

目的:研究第10号染色体同源丢失性磷酸酶-张力蛋白基因(PTEN)、抗凋亡基因survivin与基因金属基质蛋白酶-2(MMP-2)蛋白在卵巢浆液性乳头状囊腺癌(卵巢浆乳癌)中的表达及临床意义。方法:采用免疫组化SP法检测PTEN、survivin、MMP-2蛋白在36例卵巢浆乳癌、12例浆液性囊腺瘤及16例正常卵巢组织中的表达。结果:PTEN、survivin、MMP-2蛋白在卵巢浆乳癌中阳性表达率分别为38.9%、77.8%、61.1%。卵巢浆乳癌中PTEN阳性表达(38.9%)明显低于浆液性囊腺瘤组(83.3%)及正常对照组(87.5%),survivin、MMP-2的阳性表达率明显高于浆液性囊腺瘤组及正常对照组,差异有统计学意义(P<0.05),正常对照组与浆液性囊腺瘤组间的差异无统计学意义(P>0.05)。PTEN在卵巢浆乳癌Ⅰ～Ⅱ期阳性表达明显高于Ⅲ～Ⅳ期,高、中分化组明显高于低分化组,无淋巴结转移组明显高于淋巴结转移组,差异有统计学意义(P<0.05);PTEN与卵巢浆乳癌临床分期、组织分化程度呈显著负相关(P<0.05);survivin、MMP-2在Ⅲ～Ⅳ期的阳性表达高于Ⅰ～Ⅱ期,低分化组高于高、中分化组,有淋巴结转移组高于无淋巴结转移组,差异有统计学意义(P<0.05),二者与临床分期、组织分化程度呈显著正相关(P<0.05)。PTEN与survivin呈明显负相关(r=-0.573,P<0.05),survivin与MMP-2明显正相关(r=0.573,P<0.05)。结论:联合检测PTEN、survivin、MMP-2对于评价卵巢浆乳癌的恶性程度、转移及预后更具有临床价值。

Objective：To study the expression and its clinical significance of PTEN, survMn, and MMP-2 in ovarian serous cystadenocarcinomas.Methods： Immunohistochemistry method was used to determine the expression of PTEN, survivin, and MMP-2 in 36 cases of ovarian serous corpora mammillaria cystadenocarcinoma, 12 cases of serous cystadenoma, and 16 cases of normal ovarian tissues. Reaults：The positive expression rates of PTEN, survivn, and MMP-2 in ovarian cancer tissues were 38.9% （14/38）, 77.8% （28/36）, 61.1% （22/36）, respectively. The positive expression of PTEN in ovarian cancer was significantly lower than benign ovarian tumors and normal ovarian tissues, the positive expression of survivin and MMP-2 in ovarian cancer were higher than the others （ P 〈 0.05）. However there were no significant difference between normal ovarian tissues and benign ovarian tumors in each factor （ P〉0.05）. In ovarian cancer, the positive expression of PTEN in Ⅰ-Ⅱ stage was markedly higher than Ⅲ-Ⅳ stage （ P 〈 0.05）, and its positive expression in high-, middle-grade were significantly higher than the low- grade （ P 〈 0.05）, and its expression without lymph metastasis group was significantly higher than with lymph metastasis group （ P〈 0.05）. PTEN had a negative correlation with clinical stage and histologic grade. The positive expression of survivin, MMP-2 in Ⅲ-Ⅳstage was markedly higher than Ⅰ-Ⅱ stage （P〈0.05）, and their positive expressions in the low grade were significantly higher than the high-, middle-grade（ P 〈 0.05）, and their expressions in lymph metastasis groups were significantly higher than those without lymph metastasis groups （P〈0.05）. Survivin and MMP-2 had positive correlation with clinical stage and histologic grade. PTEN had negative correlation with survivin （ r = - 0.573, P 〈 0.05）, otherwise survivin had positive correlation with MMP-2 （ r = 0.573, P 〈 0.05）. Conclusions..Associated determination of PTEN, survivin, and MMP-2 will be valuable to estimate malignant, metastasis, and prognosis.