黄韧带退变早期的超微结构实验研究

Experimental studies on ultramicrostructure of ligamentum flavum at early degeneration

[目的]通过对建立的动力失衡性大鼠颈椎模型的研究,观察早期退变黄韧带超微结构的变化规律。[方法]制作动力失衡性大鼠颈椎模型,术后3d、5d、15d、30d、60d共5个时间点分别取材,透射电镜观察。[结果]颈后肌肉切除术后3d,黄韧带中出现大量的成纤维细胞。术后5d,成纤维细胞代谢活跃,成纤维细胞周围胶原纤维明显增多。术后15d,成纤维细胞减少,纤维间隙内有较多的基质成分。术后30d,成纤维细胞细长,纤维束粗大呈波浪状。术后60d,成纤维细胞数量及形态接近纤维细胞,纤维束排列规整粗大。[结论]颈椎动力失衡性大鼠黄韧带中成纤维细胞数量和胶原纤维随着术后时间的延长呈现规律性的变化。

[Objective] To study the microstructure of ligamentum fiavum of the early dynamic imbalanced rats. [Methods] The dynamic imbalanced rat mode was established. The samples were taken and studied by electron microscopy at 3, 5, 15, 30, 60 days after operation. [ Results] A large number of fibroblasts in the ligamentum flavum were found at 3 days after the neck muscles were resected. The fibroblasts had an active metabolism, and around them the collagen fibers were markedly increased at 5 days. The number of fibroblasts was reduced, but the matrices were increased at 15 days. At 30 days, fibroblasts became slender, and fibers looked like waves. At 60 days, the number and appearance of the fibroblasts of the ligamentum fiavum were similar to that of the control group. The fibers were regularly arranged and became larger than that of the colhrol group. [ Conclusion] The number of fibroblasts and the collagen fibers in the ligamentum flavum of the dynamic imbanlanced rats has a regular change after operation.