摘要
目的：观察左旋多巴对大鼠脑内5羟色胺（5-HT）能神经元的影响。方法：左旋多巴大鼠灌胃，以免疫细胞化学和流式细胞术显示5-HT及色氨酸羟化酶（TPH）免疫反应物质或含量的变化。结果：免疫细胞化学显示左旋多巴组大鼠中缝背核5-HT能神经元细胞直径明显小于对照组；中缝背核5-HT和TPH免疫反应物质灰度值比对照组分别增高了7．72％和16．57％。流式细胞术显示左旋多巴组5-HT的表达量比对照组减少了18．45％，TPH表达量比对照组减少了19．90％。结论：左旋多巴降低脑内5-HT能神经元5-HT和TPH的表达。
Objective： To study the effect of L-dopa on the serotonergic neurons in the rat brain. Methods： Wistar rats were treated with L-dopa by intragastric administration. Immunocytochemistry and flow cytometry were mainly used to analyze the altered expression of serotonin （5-HT） and tryptophan hydroxylase （TPH） in L-dopa-treated rats. Results： Immunohistochemistry showed that the diameter of 5-HT neurons in dorsal raphe nucleus （DRN） was smaller than that in control and the mean gray value of 5-HT and TPH in DRN of the L-dopatreated rats increased by 7.72% and 16.57% respectively. Flow cytometry showed that the expression of 5-HT and TPH in DRN of the L-dopa-treated rats significantly decreased by 18. 45 % and 19. 90% respectively compared with the corresponding control. Conclusion： L-dopa decreases the expression of 5-HT and TPH in the rat brain.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2009年第4期479-481,526,共4页
Chinese Journal of Anatomy
基金
河北省自然科学基金（C200900L094）
河北省教育厅计划攻关项目（2007129）
