表没食子儿茶素没食子酸酯诱导HL60细胞程序性死亡

Induction of apoptosis by (-)epigallocatechin gallate in HL60 cells

目的：探讨儿茶素类化合物的抗癌作用机制。方法：以儿茶素的主要成分表没食子儿茶素没食子酸酯（ＥＧＣＧ）为实验材料，使用噻唑蓝（ＭＴＴ）还原法，ＤＮＡ凝胶电泳，透射电镜技术，观察了ＥＧＣＧ对人急性早幼粒白血病细胞（ＨＬ６０）程序性死亡的影响。结果：０．５５×１０－３ｍｏｌ·Ｌ－１ＥＧＣＧ作用５ｈ后，琼脂糖凝胶电泳下，ＨＬ６０细胞呈现典型的ＤＮＡ条带，透射电镜观察到凋亡小体。

OBJECTIVE: To study the anticarcinogenic mechanism of polyphenolic compounds in tea. METHODS: The progammed cell death (PCD) of induction effects of (-)epigallocatechin gallate (EGCG), and the main constituent of polyphenolic compounds were observed on the human promyelocytic leukemic cell (HL60) by MTT reduction test, DNA gel electrophoresis and transmission electronic microscopy technique. RESULTS: After treating HL60 cells with EGCG in 0.55 mmol·L-1 for 5 h, DNA extracted from HL60 cells showed typical internucleosomal DNA degradation, i.e., DNA ladder and apoptotic vehicles. The effect of EGCG was shown in parallel with its cytotoxic activity in HL60 cells. CONCLUSION: These results suggest that the mechanism of antitumor action of EGCG is related to its PCD inducing activity.