西洋参多糖5-2的分离、性质和活性研究

Isolation, properties and bioactivities of polysaccharide 52 fromPanax quinquefolium

目的：研究西洋参多糖（ＰＰＱ）中均一组分ＰＰＱ５２的分离、糖组成、相对分子量及生物活性。方法：ＳｅｐｈａｄｅｘＤＥＡＥＡ５０和ＳｅｐｈａｄｅｘＧ１００分离，气相色谱法测定糖组成，基底辅助激光解吸／电离质谱测定相对分子量。用小鼠脾淋巴细胞进行淋巴细胞转化实验和白介素２促诱生实验。结果：ＰＰＱ５２由Ｄ阿拉伯糖和Ｄ半乳糖以１∶１．１摩尔比组成，含糖醛酸，相对分子量２１０００Ｄ，具有刺激淋巴细胞转化及白介素２促诱生功能。

OBJECTIVE: To isolate bioactive polysaccharides from [WTBX]Panax quinquefolium[WTBZ] L. and determine its sugar composition, uronic acid content, molecular weight and biological activities. METHODS: Sephadex DEAE A50 and Sephadex G100 chromatography were used. The sugar composition was analyzed by gas chromatography. The molecular weight was determined by matrix assisted laser desorption/ionization mass spectrometer (MALDIMS). RESULTS: Isolated PPQ52 was composed of Darabinose and Dgalactose in a molar ratio of 1∶1.1. It contained uronic acid. The average molecular weight was 21 000 Dalton. It stimulated the transformation of lymphocyte and induced the production of IL2. CONCLUSION: Immunologically active ploysaccharide could be obtained from [WTBX]Panax quinquefolium L.