摘要
目的:研究西洋参多糖(PPQ)中均一组分PPQ52的分离、糖组成、相对分子量及生物活性。方法:SephadexDEAEA50和SephadexG100分离,气相色谱法测定糖组成,基底辅助激光解吸/电离质谱测定相对分子量。用小鼠脾淋巴细胞进行淋巴细胞转化实验和白介素2促诱生实验。结果:PPQ52由D阿拉伯糖和D半乳糖以1∶1.1摩尔比组成,含糖醛酸,相对分子量21000D,具有刺激淋巴细胞转化及白介素2促诱生功能。
OBJECTIVE: To isolate bioactive polysaccharides from [WTBX]Panax quinquefolium[WTBZ] L. and determine its sugar composition, uronic acid content, molecular weight and biological activities. METHODS: Sephadex DEAE A50 and Sephadex G100 chromatography were used. The sugar composition was analyzed by gas chromatography. The molecular weight was determined by matrix assisted laser desorption/ionization mass spectrometer (MALDIMS). RESULTS: Isolated PPQ52 was composed of Darabinose and Dgalactose in a molar ratio of 1∶1.1. It contained uronic acid. The average molecular weight was 21 000 Dalton. It stimulated the transformation of lymphocyte and induced the production of IL2. CONCLUSION: Immunologically active ploysaccharide could be obtained from [WTBX]Panax quinquefolium L.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1998年第8期494-496,共3页
Chinese Pharmaceutical Journal
关键词
西洋参
多糖
激光解吸
电离质谱
分离
Panax quinquefolium L., polysaccharide, matrix assisted laser desorption/ionization mass spectrometry, interleukin2