电针“大椎”“命门”对佐剂性关节炎大鼠抗炎及免疫调节反应的影响

Influence of Eiectroacupuncture of"Dazhui"(GV 14)"Mingmen"(GV 4) and Non-acupoint on the Inflammation and Immune Reactions in Adjuvant Arthritis Rats

目的:观察并比较电针"大椎""命门"对佐剂性关节炎大鼠免疫调节作用的影响。方法:40只Wistar大鼠随机分为正常组、模型组、大椎组、命门组、尾部中点组,每组8只,注射弗氏完全佐剂造佐剂性关节炎大鼠模型。电针"大椎""命门"及尾部中点,每次20min,隔日1次,共8次。观察治疗前后大鼠足跖肿胀度,使用MTT比色法检测脾脏淋巴细胞增殖率、白介素(IL)-2活性,放射免疫法检测血清IL-2、肿瘤坏死因子(TNF)-α含量。结果:大椎组、命门组的T、B淋巴细胞增殖率,脾IL-2活性,血清IL-2含量及尾部中点组T、B淋巴细胞增殖率,脾IL-2活性与模型组比较明显升高(P<0.01),大椎组血清TNF-α含量与模型组比较降低(P<0.05),大椎组脾IL-2活性及命门组T、B淋巴细胞增殖率,脾IL-2活性与尾部中点组比较升高(P<0.05)。结论:电针通过对T、B淋巴细胞增殖率,IL-2,TNF-α的相互调节,起到抗炎免疫调节作用。

Objective To observe the effect of electroacupuncture （EA） on the levels of lymphocyte proliferation rate, spleen and serum interleukin-2 （IL-2）, serum tumor necrosis faetor-α（TNF-α, so as to analyze its immune-regulative function in adjuvant arthritis （AA） rats. Methods A total of 40 Wistar rats were randomized into normal control, model, Dazhui （GV 14）, Mingmen （GV 4） and non-acupoint groups. Adjuvant arthritis model was established by subcutaneous injection of Freund＇s com- plete adjuvant into the paw. EA was applied to ＂Dazhui＂ （GV 14）, ＂Mingmen＂ （GV 4） and non-acupoint （mid-tail） for 20 rain, once every other day, 8 times altogether. Paw volume （swelling degree of the voix pedis） was detected; T and B lymphocyte transformation rates （LTR）, and IL-2 activity of the spleen tissue were assayed with MTT chromatometry, and serum IL-2 and TNF-αcontents were determined by radioimmunoassay. Results Compared to normal group, the paw volume and serum TNF-αcontent of model group increased significantly （P〈0.01, P〈0.05）, and T- and B-lymphocyte proliferation rates and spleen IL-2 activity in model group decreased considerably （P〈0.01）. In comparison with model group, paw volumes of GV 14, GV 4, and non-acupoint groups and serum TNF-αcontent of GV 14 group lowered significantly （ P〈0.01, P〈0.05） ; while T- and B-lympho- cyte proliferation rates, spleen IL-2 activity in GV 14, GV 4, and non-acupoint groups, as well as serum IL-2 content in GV 14 and GV 4 groups increased remarkably（ P〈0.01 ）. Paw volumes of both GV 14 and GV 4 groups were significantly lower than that of non-acupoint group （P〈0.05）; T- and B-lymphocyte proliferation rates and IL-2 activity of GV 4 group were significantly higher than those of non-acupoint group （P〈0.05）. No significant differences were found between GV 14 and GV 4 groups in paw vol- ume, T- and B-lymphocyte proliferation rates, spleen IL-2 activity, serum IL-2 and TNF-αcontents, and between GV 14 and non- acupoint groups in T- and B-lymphocyte proliferation rates, as well as among GV 14, GV 4 and non-acupoint groups in serum IL-2 and TNF-α contents （P〉0.05）. Conclusion Electroacupuncture of GV 14 and GV 4 induced immunoregulation on lymphocyte, L-2 and TNF-α may contribute to its anti-inflammation effect in AA rats