家蚕传染性软化病病毒的冷冻电子显微镜三维重构

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摘要应用冷冻电子显微镜三维重构技术获得了家蚕传染性软化病病毒(Infectious flacherie virus,IFV)衣壳的三维立体结构.重构最终结果使用了5047个病毒粒子的数据.FSC曲线显示该重构结果的分辨率为18?.IFV的衣壳直径为302.4?,遵循拟T=3(P=3)二十面体对称.衣壳为单层,厚度15?,表面光滑致密,无明显突起或凹陷,无孔洞贯穿.将IFV衣壳结构与昆虫的类小RNA病毒-蟋蟀麻痹病毒(Cricket paralysis virus,CrPV)以及人小RNA病毒-人鼻病毒14(human rhinovirus14,HRV14)的衣壳进行比较,发现IFV衣壳结构与CrPV更为接近.与CrPV一样,IFV衣壳表面也缺少"Rossmann峡谷".同时预测了IFV结构蛋白VP2和VP3的肽链折叠拓扑结构,并对亚基在衣壳表面的分布位置进行了推测.

作者谢礼张勤奋鲁兴萌戴兴红李鲲鹏洪健周雪平

机构地区浙江大学生物技术研究所中山大学生命科学学院浙江大学动物科学学院浙江大学分析测试中心

出处《中国科学（C辑）》 CSCD 北大核心 2009年第8期768-774,共7页 Science in China(Series C)

基金国家自然科学基金(批准号:30370305) 国家重点基础研究发展计划(批准号:2005CB121003)资助项目

关键词家蚕传染性软化病病毒(Infectious FLACHERIE virus) 冷冻电子显微镜三维重构肽链折叠

分类号 S884 [农业科学—特种经济动物饲养]

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参考文献27

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共引文献32

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家蚕传染性软化病病毒的冷冻电子显微镜三维重构

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