枳实类黄酮体外抗氧化作用研究

Antioxidation in vitro of Flavonoids from Frutus aurantii immatures

用70%乙醇微波中火提取干燥的脱脂枳实粉1.5 min,浓缩干燥得枳实粗提物。提取液过D101大孔树脂,将洗脱液浓缩干燥得枳实纯化物,经测定总黄酮达到92.78%,HPLC测其黄烷酮含量,并测定各提取物的抗氧化活性。结果显示,枳实粗提物、纯化物及黄酮单体均对羟自由基(.OH)、2,2-二苯代苦味酰基自由基(DPPH.)有清除效果,并随着浓度的增加,其对自由基的清除作用呈正相关。提取物对超氧阴离子自由基(.O2-)的清除效果在一定范围内上下波动。枳实提取物具有较强的抗氧化作用,单体之间存在协同作用。

Deffatted dry powder of Frutus aurantii was extracted with 70 % ethanol under microwave irradiation at the ratio of solvent to material 40： 1, microwave power 385 W, and extracting time 1.5 rain. The crude extract was obtained ＇after the solution was filtered,concentrated and dried. The purified extract was obtained using D101 macroporous resin, then concentrated and dried. The content of flavonoids was detected by HPLC. The scavenging activities of the crude extract, the purified extract and fla- vonoids toward hydroxyl free radical （.OH） ,DPPH free radical （DPPH-） and superoxide anion free radical （·02-） were analyzed. The results showed that extracts and flavonoids possessed the dosage effects on scavenging ·OH, DPPH· and ·O2^-. The extracts from brutus aurantii immaturus possess quite strong antioxidative activities. There are some synergistic effects among the monomers.