摘要
对从松针中提取多糖的工艺条件进行了研究。在提取过程中,比较了水、醇两种溶剂对3种松针提取的多糖得率的影响。并通过单因素法分析了5个主要因素:料液比、提取温度、提取时间、pH值及提取次数对湿地松松针水提多糖得率的影响。在单因素试验的基础上,通过正交试验设计方法对水提湿地松松针多糖的工艺条件进行了优化。试验表明,湿地松松针多糖的最佳提取工艺条件为:料液比1∶14(g∶mL),提取温度100℃,浸提时间3 h,pH值6。在此最佳工艺条件下,湿地松松针多糖得率为4.024%±0.017%。
Process of extracting polysaceharides from 3 kinds of pine needles has been studied. During the extracting process, the contents of polysaceharides of three pine needles were compared between sovents of water and ethanol. Five main factors including ratio of solid to liquid, extracting temperature, extracting time, pH value and extraction times were studied on the yield of pine needles polysaccharides. Using single factor analysis, the optimal processing conditions for water extraction of polysaeeharides were obtained by orthogonal experiments. The results showed that the optimum parameters were：extraction time 3 h at 100 ℃ with 1 ： 14 ratio of solid to liquid,and pH value 6, and the yield of polysaeeharides of P. elliottii was 4. 024 %±0. 017 %.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2009年第4期73-76,共4页
Chemistry and Industry of Forest Products
基金
浙江省科技计划项目(2007C32039)
浙江省教育厅科研项目(20060490)
关键词
湿地松
松针
多糖
工艺优化
正交试验
Pinus elliottii Engelm.
pine needles
polysaccharides
optimization
orthogonal experiments
